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Testing Equine Serum for IgG
Roche Cobas Mira (with dilution software) Parameters
| Materials Required |
| 1. Polymer Buffer | Part # 9000 |
| 2. Goat anti-Equine IgG Serum | Part # 75501 |
| 3. Equine IgG Calibrator | Part # 2002 |
| 4. Equine Serum Control | Part # 2010 |
| 5. Saline | |
Calibrator Preparation |
| Std 5 | Neat | 100ml Equine IgG Cal | Part # 2002 |
| Std 4 | 1:2 | 100ml Std 5 + 100ml Saline | |
| Std 3 | 1:4 | 100ml Std 4 + 100ml Saline | |
| Std 2 | 1:8 | 100ml Std 3 + 100ml Saline | |
| Std 1 | 1:16 | 100ml Std 2 + 100ml Saline | |
Parameters
| GENERAL | | CONVERS. | FACTOR: | 1.00000 |
| MEASUREMENT MODE: | ABSORB | | OFFSET: | 0.00000 |
| REACTION MODE: | D-R-S-SR1 | | | |
| CALIBRATION MODE: | LOGIT/LOG4 | TEST RANGE | LOW: | OFF |
| REAGENT BLANK: | REAG/DIL | | HIGH: | OFF |
| CLEANER: | NO | NORM. RANGE | LOW: | NO |
| | | HIGH: | NO | |
| WAVELENGTH: | 340nm | | | | |
| DECIMAL POSITION: | 1 | NUMBER OF STEPS: | 1 |
| UNIT: | mg/dl | | | |
| | CALC. STEP A: | | ENDPOINT |
| ANALYSIS | | READINGS | FIRST: | 1 |
| | | LAST: | 13 |
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| 5. DILUTION | NAME: | SALD | CALIBRATION | |
| | FACTOR: | 11.00 | CALIB. INTERVAL: | ON REQUEST |
| | TIME: | NO | | | |
| | STD: | FACTOR | BLANK | | |
| POST DIL. FACTOR: | 1.50 | REAG. RANGE | LOW: | NO |
| CONC. FACTOR: | 6.00 | | HIGH: | NO |
| SAMPLE | CYCLE: | 1 | BLANK RANGE | LOW: | NO |
| VOLUME: | 4.0mL | | HIGH: | NO |
| DILUTION NAME: | H2O | 3. STANDARD | POS: |
| | VOLUME: | 15.0mL | | | |
| 1. REAGENT | CYCLE: | 1 | | | |
| VOLUME: | 200mL | REPLICATE: | DUPL | |
| 2. START R1 | CYCLE: | 2 | DEVIATION: | NO | |
| VOLUME: | 20.0mL | CORRECTION STD: | NO | |
| DILUTION NAME: | H2O | | | |
| | VOLUME: | 10.0mL | 4. CONTROL | | |
| CALCULATION: | CS1 POS: | LOW: | |
| SAMPLE | LIMIT: | 0.1000 | | ASSIGN: | |
| POINT : | 1 | | HIGH: | |
| REAC. DIRECTION: | INCREASE | | | |
| | CHECK: | ON | CS2 POS: | NO |
| ANTIGEN | EXCESS: | 80% | CS3 POS: | NO | |
| | POINT: | 5 | | | |
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Rev. Date: May 14, 2004
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